Could not get any result from my IDBA-UD contig files generated from Shogun metagenomic data

[shankhanath]

Hi,

I have successfully installed VIBRANT 1.2.1.
I have several skin shotgun metagenomic data (human reads removed) and I have used IDBA-UD to generate contig.fa and scaffolds.fa from those shotgun data. I ran the code to get the output

python3 /data/sata_data/home/souvik_p1/VIBRANT/VIBRANT_run.py -i contig.fa

python3 /data/sata_data/home/souvik_p1/VIBRANT/VIBRANT_run.py -i scaffolds.fa

There was no error observed.

I did not get any results. VIBRANT_phages folder gave all the files with zero size. I have used several samples now. But still not getting the data.

In parallel I have used virsorter2 from contig.fa it gave many viral contigs. I dids BLAST (against NCBI Viral RefSeq database) using the virsorter contig. It identified bacteriophages. I have used these viral contig files also in VIBRANT but did not get any results. I have attached all the fasta files here also.
Fasta_files.zip

To further understand I downloaded a bacteriophage fasta Staphylococcus phage StB12 from ENA and ran the code. but it also did not gave any results. All the files generated with zero output. [https://www.ebi.ac.uk/ena/browser/view/JN700520]

python3 /data/sata_data/home/souvik_p1/VIBRANT/VIBRANT_run.py -i JN700520.2.fasta

Please help me

[lizzi-coy]

Did you solve this issue? I am having the same problem