Hi, I’m wondering about the v-score cutoff used to eliminate false positives. From the manuscript, I understand that v-scores of 1 and above are assigned to sequences containing viral hallmark proteins, while non-viral sequences will have low v-scores. But how low should the v-score be to consider a sequence non-viral? I used VIBRANT on metagenomic samples with the default settings, and some of the viral sequences predicted by VIBRANT have a v-score of 0.2. However, the same sequences were not identified as viral by Genomad or Virsorter2.
Hi, I’m wondering about the v-score cutoff used to eliminate false positives. From the manuscript, I understand that v-scores of 1 and above are assigned to sequences containing viral hallmark proteins, while non-viral sequences will have low v-scores. But how low should the v-score be to consider a sequence non-viral? I used VIBRANT on metagenomic samples with the default settings, and some of the viral sequences predicted by VIBRANT have a v-score of 0.2. However, the same sequences were not identified as viral by Genomad or Virsorter2.