@@ -324,25 +324,25 @@ USAGE(S):
324324 rAMPage.sh [-a <address>] [-b] [-c <taxonomic class>] [-d] [-f] [-h] [-m] [-n <species name>] [-o <output directory>] [-p] [-r <FASTA.gz>] [-s] [-t <int>] [-v] <input reads TXT file>
325325
326326OPTIONS:
327- -a <address> email address for alerts
328- -b benchmark (uses /usr/bin/time -pv)
329- -c <class> taxonomic class of the dataset (default = top-level directory in $outdir)
330- -d debug mode of Makefile
331- -f force characterization even if no AMPs found
332- -h show help menu
333- -m <target > Makefile target (default = exonerate )
334- -n <species > taxonomic species or name of the dataset (default = second-level directory in $outdir )
335- -o <directory> output directory (default = directory of input reads TXT file)
336- -p run processes in parallel
337- -r <FASTA.gz> reference transcriptome (accepted multiple times, *.fna.gz *.fsa_nt.gz )
338- -s strand-specific library construction (default = false )
339- -t <int> number of threads (default = 48)
340- -v print version number
341- -E <e-value > E-value threshold for homology search (default = 1e-5 )
342- -S <0 to 1> AMPlify score threshold for amphibian AMPs (default = 0.90 )
343- -L <int> Length threshold for AMPs (default = 30 )
344- -C <int> Charge threshold for AMPs (default = 2)
345-
327+ -a <address> email address for alerts
328+ -c <class> taxonomic class of the dataset (default = top-level directory in $outdir)
329+ -d debug mode of Makefile
330+ -f force characterization even if no AMPs found
331+ -h show help menu
332+ -m <target> Makefile target (default = exonerate)
333+ -n <species > taxonomic species or name of the dataset (default = second-level directory in $outdir )
334+ -o <directory > output directory (default = directory of input reads TXT file )
335+ -p run processes in parallel
336+ -r <FASTA.gz> reference transcriptome (accepted multiple times, *.fna.gz *.fsa_nt.gz)
337+ -s strand-specific library construction (default = false )
338+ -t <int> number of threads (default = 48 )
339+ -v print version number
340+ -E <e-value> E-value threshold for homology search (default = 1e-5)
341+ -S <0 to 1 > AMPlify score threshold for amphibian AMPs (default = 0.90 )
342+ -L <int> Length threshold for AMPs (default = 30 )
343+ -C <int> Charge threshold for AMPs (default = 2 )
344+ -R Disable redundancy removal during transcript assembly
345+
346346EXAMPLE(S):
347347 rAMPage.sh -a user@example.com -c class -n species -p -s -t 8 -o /path/to/output/directory -r /path/to/reference.fna.gz -r /path/to/reference.fsa_nt.gz /path/to/input.txt
348348
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