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Run alternative_splicing_events on filtered transcripts #30

@IngoMue

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@IngoMue

Dear IsoTools team,

Thanks a lot for creating isotools, it seems perfect to analyse our isoseq data.

I was just wondering, if it is somehow possible to run alternative_splicing_events on filtered transcripts?
I'm mainly following the steps described in your tutorial and/or case studies, but I'm missing on whether filters are automatically applied during certain steps.
Besides filters on coverage and read fraction for the alternative, I can only see that alternative_splicing_events supports filters on the gene context (through iter_genes).
Since a significant number of reads across our samples fall into the categories of the BALANCED filter (~17% Internal priming, ~13% fragments and ~2.5% RTTS), I would like to exclude these before detecting AS events.

Thanks a lot for your help in advance!
Ingo

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