Dear IsoTools team,
Thanks a lot for creating isotools, it seems perfect to analyse our isoseq data.
I was just wondering, if it is somehow possible to run alternative_splicing_events on filtered transcripts?
I'm mainly following the steps described in your tutorial and/or case studies, but I'm missing on whether filters are automatically applied during certain steps.
Besides filters on coverage and read fraction for the alternative, I can only see that alternative_splicing_events supports filters on the gene context (through iter_genes).
Since a significant number of reads across our samples fall into the categories of the BALANCED filter (~17% Internal priming, ~13% fragments and ~2.5% RTTS), I would like to exclude these before detecting AS events.
Thanks a lot for your help in advance!
Ingo
Dear IsoTools team,
Thanks a lot for creating isotools, it seems perfect to analyse our isoseq data.
I was just wondering, if it is somehow possible to run
alternative_splicing_eventson filtered transcripts?I'm mainly following the steps described in your tutorial and/or case studies, but I'm missing on whether filters are automatically applied during certain steps.
Besides filters on coverage and read fraction for the alternative, I can only see that
alternative_splicing_eventssupports filters on the gene context (throughiter_genes).Since a significant number of reads across our samples fall into the categories of the
BALANCEDfilter (~17% Internal priming, ~13% fragments and ~2.5% RTTS), I would like to exclude these before detecting AS events.Thanks a lot for your help in advance!
Ingo