Suggestion: Use of predefined cell localisation instead of DAPI image

[GBeattie]

Hey,

Great looking package, like the approach!

It's possible I'm missing a more fundamental need for the DAPI image, but if it's only use is to assign transcripts to the nuclei this information is also included in the transcript files (at least from CosMx). If the need for a raw DAPI image could be bypassed in cases where transcript localisation has already been determined, this would streamline the package quite a bit and negate the need for downloading/handling burdensome raw images.

All the best,
Gordon

[xhelenfu]

Hi Gordon, thanks for the suggestion. The nuclei masks are also used during the prediction step of the cell masks. So we work from the DAPI image to help ensure consistency of the nuclei through the pipeline