diff --git a/CHANGELOG.md b/CHANGELOG.md
index 5e177a1..3248579 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -11,6 +11,10 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 - QIDO-RS and MWL services now support `uid-list-matching` syntax for match query parameters. 
 
+### Changed
+
+- Trailing slashes in URLs are now trimmed for all endpoints before processing (`/studies/` and `/studies` are equivalent).
+
 ## [0.3.0]
 
 ### Added
diff --git a/Cargo.toml b/Cargo.toml
index 57331e7..26f0a32 100644
--- a/Cargo.toml
+++ b/Cargo.toml
@@ -42,7 +42,7 @@ axum-extra = { version = "0.10.3", features = ["query"] }
 axum-streams = { version = "0.23.1", features = ["json"] }
 futures = "0.3.31"
 mime = "0.3.17"
-tower-http = { version = "0.6.6", features = ["trace", "cors", "timeout"] }
+tower-http = { version = "0.6.6", features = ["trace", "cors", "timeout", "normalize-path"] }
 tower = { version = "0.5.2", features = ["limit"] }
 url = "2.5.7"
 async-trait = "0.1.89"
diff --git a/src/main.rs b/src/main.rs
index 74413bb..e326254 100644
--- a/src/main.rs
+++ b/src/main.rs
@@ -13,11 +13,15 @@ use crate::types::AE;
 use association::pool::AssociationPools;
 use axum::extract::{DefaultBodyLimit, Request};
 use axum::response::Response;
+use axum::routing::IntoMakeService;
+use axum::ServiceExt;
 use std::net::SocketAddr;
 use std::time::Duration;
 use tokio::net::TcpListener;
 use tokio::signal;
+use tower::Layer;
 use tower_http::cors::CorsLayer;
+use tower_http::normalize_path::NormalizePathLayer;
 use tower_http::timeout::TimeoutLayer;
 use tower_http::trace;
 use tracing::{error, info, level_filters::LevelFilter, Level};
@@ -144,6 +148,9 @@ async fn run(config: AppConfig) -> anyhow::Result<()> {
 		)))
 		.with_state(app_state);
 
+	let app = NormalizePathLayer::trim_trailing_slash().layer(app);
+	let service = ServiceExt::<Request>::into_make_service(app);
+
 	let HttpServerConfig {
 		interface: host,
 		port,
@@ -159,11 +166,11 @@ async fn run(config: AppConfig) -> anyhow::Result<()> {
 		"Started DICOMweb server"
 	);
 	if config.server.http.graceful_shutdown {
-		axum::serve(listener, app)
+		axum::serve(listener, service)
 			.with_graceful_shutdown(shutdown_signal())
 			.await?;
 	} else {
-		axum::serve(listener, app).await?;
+		axum::serve(listener, service).await?;
 	}
 
 	Ok(())