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<!DOCTYPE html>
<html lang="en">
<head>
<meta charset="utf-8">
<meta http-equiv="X-UA-Compatible" content="IE=edge">
<meta name="viewport" content="width=device-width, initial-scale=1">
<meta name="description" content="">
<meta name="author" content="">
<title>BMW CV</title>
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<body id="page-top" class="cv">
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<a class="navbar-brand" href="index.html"><span class="icon-home"></span> <span class="noDisplay">Blake Williams</span></a>
</div>
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<li class="page-scroll">
<a href="#skills">Skills</a>
</li>
<li class="page-scroll">
<a href="#proXP">Professional Experience</a>
</li>
<li class="page-scroll">
<a href="#education">Education</a>
</li>
<li class="page-scroll">
<a href="#publications">Publications</a>
</li>
<li class="page-scroll">
<a href="#contact">Contact</a>
</li>
</ul>
</div>
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</div>
<!-- /.container-fluid -->
</nav>
<!-- Intro Header -->
<header class="intro">
<div class="intro-body">
<div class="container darkHead">
<div class="row">
<div class="col-lg-8 col-lg-offset-2">
<h1 class="brand-heading">Humblebrag</h1>
<p class="intro-text">I'm a creative, independent, MS accredited Bioengineer with hands-on experience in molecular biology techniques, troubleshooting and experimental design.</p>
<li class="page-scroll">
<a href="#skills" class="btn btn-circle">
<i class="icon-angle-double-down animated"></i>
</a>
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</header>
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<section id="skills">
<div class="container">
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<div class="col-lg-12 text-center">
<h2>Skills</h2>
<hr class="star-primary lightning">
<br>
</div>
<div class="col-sm-4 text-center">
<span class="icon icon-stats"></span>
<br>
<br>
<p>Assay development</p>
<p>Experimental design</p>
<p>Project management</p>
<p>Data analysis</p>
<p>Troubleshooting</p>
<br>
</div>
<div class="col-sm-4 text-center">
<span class="icon icon-lab"></span>
<br>
<br>
<p>Protein purification</p>
<p>Protein characterization</p>
<p>Cell culture</p>
<p>Molecular cloning</p>
<p>PCR</p>
<br>
</div>
<div class="col-sm-4 text-center">
<span class="icon icon-keyboard"></span>
<br>
<br>
<p>Matlab</p>
<p>Solidworks</p>
</div>
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<div class="row">
<div class="col-md-6 col-sm-12">
<h3>Research Assistant</h3>
<p>Applied the methodology of reverse protein engineering to develop a fluorescent peptide</p>
<p>Engineered an orthogonal tRNA and tRNA synthetase pair for site-specific incorporation of unnatural amino acids into proteins in mammalian cells</p>
<p>Detected protein-protein interactions using the tetracycline repressor-based mammalian two-hybrid system</p>
<p>Evaluated the purity of protein samples isolated from <i>E.coli</i> using FPLC, SDS-PAGE, and western blot</p>
<p>Developed assays to characterize the functionality of novel proteins and peptides</p>
<p>Applied troubleshooting skills to successfully identify solutions to problems encountered during experiments</p>
<p class="time"><br>2011 - Current</p>
</div>
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<img data-scroll-reveal="enter right and move 50px over 1.33s" class="img-responsive noMove" src="img/goldenGate-1.png" alt="">
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<div class="col-md-8 col-md-offset-2">
<p>Planned a series of experiments and designed protocols for three new courses: Biomolecular Engineering I & II, and Bio-device Engineering</p>
<p>Explained the principles of GLP and demonstrated basic lab techniques to undergraduates</p>
<p>Advised senior thesis research and developed experimental designs for students to follow</p>
<p>Managed the experiments of graduate and undergraduate students to efficiently obtain results</p>
<p>Conducted brief presentations at the beginning of each lab session to explain information and concepts related to the experiments</p>
<p class="time"><br>2012 - Current</p>
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</section>
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<h3>Research Assistant Internship</h3>
<p>Tested the potency of synthetic inhibitor candidates for Sortase A</p>
<p>Conducted activity screening assays and successfully purified Sortase A</p>
<p>Attended lectures and training sessions to complete the International Summer Academy of Pharmacy program</p>
<p class="time"><br>Summer 2013</p>
</div>
<div class="col-md-6 col-md-pull-6 col-md-offset-0 col-xs-6 col-xs-offset-3">
<img data-scroll-reveal="enter left and move 50px over 1.33s" class="img-responsive noMove" src="img/shanghaiReal.png" alt="">
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</section>
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<h2>Education & Training</h2>
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<br>
</div>
</div>
<div class="row">
<div class="col-sm-4 text-center">
<h3>B.S. - Bioengineering</h3>
<p>Santa Clara University</p>
<p class="time">2012</p>
</div>
<div class="col-sm-4 col-sm-offset-0 col-xs-6 col-xs-offset-3">
<img class="img-responsive center-block" src="img/SCU-logo.png" alt="">
</div>
<div class="col-sm-4 col-xs-12 text-center">
<h3>M.S. - Bioengineering</h3>
<p>Santa Clara University</p>
<p class="time">2014</p>
</div>
</div>
</div>
</section>
<!-- Publications Section -->
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<h2>Publications</h2>
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<br>
</div>
</div>
<div class="row">
<div class="col-lg-12">
<h3>Articles</h3>
<p>Xiang, L., Moncivais, K., Jiang, F., Williams, B., Alfonta, L., Zhang, Z. J. <a href="http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0067333" class="linkColor">“Crucial Optimization of Translational Components toward Efficient Incorporation of Unnatural Amino Acids into Proteins in Mammalian Cells”</a> PLOS ONE, 12 July 2013.</p>
<p>Zhu, J., Xiang, L., Williams, B., Jiang, F., Zhang, Z. J. “Equilibrium of Sortase A Dimerization on Gram-Positive Bacterial Surface Mediates its Cell Sorting Activity” Journal of Biological Chemistry, in press.</p>
<p>Williams, B., Umeda, A., Shimomura, E., Chanthery, Y., Zhang, Z. J. “Devolution of GFP: Reverse Protein Engineering a Fluorescent Peptide” Science, in preparation.</p>
</div>
<div class="col-lg-12">
<h3>Patents</h3>
<p>Zhiwen Zhang, Blake Williams, Aiko Umeda. Peptidic fluorescent agents and the methodology of reverse protein engineering, U.S. Provisional Patent Application # 61/954812. Filing Date: March 18, 2014.</p>
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<p class="smallText">The form above will send a message directly to my email account. If you prefer using your own email client, email me at blakemacwilliams -AT- gmail -DOT- com.</p>
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<p class="smallText">Santa Clara, CA 95050</p>
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Made by Winston in Crown Heights in 2014
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