大佬们求助！我换了个元数据集，运行失败问题

各位大佬，我又来了，基于之前[篇章](https://github.com/jameslz/dix-seq/issues/1)，我跑起来数据结果，但我这边上网找了别的样例数据跑，又跑不起来了。

- 参考数据集文章：https://zhuanlan.zhihu.com/p/16210922715
- 参考数据集地址：https://mothur.s3.us-east-2.amazonaws.com/wiki/miseqsopdata.zip

一. 问题：
1、我现在通过检查命令是没问题的，但现在跑不起来，而且查看日志时候，trimming.txt会提示我：java.lang.RuntimeException: Invalid FASTQ name line: 错误

> Using PrefixPair: 'TACACTCTTTCCCTACACGACGCTCTTCCGATCT' and 'GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT'
Using PrefixPair: 'TACACTCTTTCCCTACACGACGCTCTTCCGATCT' and 'GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT'
Using PrefixPair: 'TACACTCTTTCCCTACACGACGCTCTTCCGATCT' and 'GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT'
Using PrefixPair: 'TACACTCTTTCCCTACACGACGCTCTTCCGATCT' and 'GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT'
Using PrefixPair: 'TACACTCTTTCCCTACACGACGCTCTTCCGATCT' and 'GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT'
Using PrefixPair: 'TACACTCTTTCCCTACACGACGCTCTTCCGATCT' and 'GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT'
Using PrefixPair: 'TACACTCTTTCCCTACACGACGCTCTTCCGATCT' and 'GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT'
Using PrefixPair: 'TACACTCTTTCCCTACACGACGCTCTTCCGATCT' and 'GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT'
ILLUMINACLIP: Using 1 prefix pairs, 0 forward/reverse sequences, 0 forward only sequences, 0 reverse only sequences
ILLUMINACLIP: Using 1 prefix pairs, 0 forward/reverse sequences, 0 forward only sequences, 0 reverse only sequences
ILLUMINACLIP: Using 1 prefix pairs, 0 forward/reverse sequences, 0 forward only sequences, 0 reverse only sequences
ILLUMINACLIP: Using 1 prefix pairs, 0 forward/reverse sequences, 0 forward only sequences, 0 reverse only sequences
ILLUMINACLIP: Using 1 prefix pairs, 0 forward/reverse sequences, 0 forward only sequences, 0 reverse only sequences
ILLUMINACLIP: Using 1 prefix pairs, 0 forward/reverse sequences, 0 forward only sequences, 0 reverse only sequences
ILLUMINACLIP: Using 1 prefix pairs, 0 forward/reverse sequences, 0 forward only sequences, 0 reverse only sequences
ILLUMINACLIP: Using 1 prefix pairs, 0 forward/reverse sequences, 0 forward only sequences, 0 reverse only sequences
Exception in thread "main" Exception in thread "main" Exception in thread "main" Exception in thread "main" Exception in thread "main" Exception in thread "main" Exception in thread "main" Exception in thread "main" java.lang.RuntimeException: Invalid FASTQ name line: F3D8S196_1.fastq                                                                                    0000755 0000000 0000000 00013255631 15035640755 012120  0                                                                                                    ustar   root                            root                                                                                                                                                                                                                   @M00967:43:000000000-A3JHG:1:1101:17304:1428 1:N:0:196
java.lang.RuntimeException: Invalid FASTQ name line: F3D2S190_1.fastq                                                                                    0000755 0000000 0000000 00052014627 15035640754 012104  0                                                                                                    ustar   root                            root                                                                                                                                                                                                                   @M00967:43:000000000-A3JHG:1:1101:17046:1548 1:N:0:190
	at org.usadellab.trimmomatic.fastq.FastqParser.parseOne(FastqParser.java:68)
java.lang.RuntimeException: Invalid FASTQ name line: F3D5S193_1.fastq                                                                                    0000755 0000000 0000000 00011415767 15035640754 012117  0                                                                                                    ustar   root                            root                                                                                                                                                                                                                   @M00967:43:000000000-A3JHG:1:1101:9945:2661 1:N:0:193
java.lang.RuntimeException: Invalid FASTQ name line: F3D6S194_1.fastq                                                                                    0000755 0000000 0000000 00021071302 15035640754 012101  0                                                                                                    ustar   root                            root                                                                                                                                                                                                                   @M00967:43:000000000-A3JHG:1:1101:16193:1426 1:N:0:194
java.lang.RuntimeException: Invalid FASTQ name line: F3D0S188_1.fastq                                                                                    0000755 0000000 0000000 00020542010 15035640752 012072  0                                                                                                    ustar   root                            root                                                                                                                                                                                                                   @M00967:43:000000000-A3JHG:1:1101:18327:1699 1:N:0:188
	at org.usadellab.trimmomatic.fastq.FastqParser.parseOne(FastqParser.java:68)
java.lang.RuntimeException: Invalid FASTQ name line: F3D7S195_1.fastq                                                                                    0000755 0000000 0000000 00012770747 15035640754 012126  0                                                                                                    ustar   root                            root                                                                                                                                                                                                                   @M00967:43:000000000-A3JHG:1:1101:18321:2202 1:N:0:195
java.lang.RuntimeException: Invalid FASTQ name line: F3D1S189_1.fastq                                                                                    0000755 0000000 0000000 00014444103 15035640753 012104  0                                                                                                    ustar   root                            root                                                                                                                                                                                                                   @M00967:43:000000000-A3JHG:1:1101:18954:2112 1:N:0:189
java.lang.RuntimeException: Invalid FASTQ name line: F3D3S191_1.fastq                                                                                    0000755 0000000 0000000 00016363420 15035640754 012104  0                                                                                                    ustar   root                            root                                                                                                                                                                                                                   @M00967:43:000000000-A3JHG:1:1101:18489:1989 1:N:0:191
	at org.usadellab.trimmomatic.fastq.FastqParser.parseOne(FastqParser.java:68)
	at org.usadellab.trimmomatic.fastq.FastqParser.parseOne(FastqParser.java:68)
	at org.usadellab.trimmomatic.fastq.FastqParser.parse(FastqParser.java:164)
	at org.usadellab.trimmomatic.fastq.FastqParser.parseOne(FastqParser.java:68)
	at org.usadellab.trimmomatic.fastq.FastqParser.parseOne(FastqParser.java:68)
	at org.usadellab.trimmomatic.fastq.FastqParser.parse(FastqParser.java:164)
	at org.usadellab.trimmomatic.fastq.FastqParser.parseOne(FastqParser.java:68)
	at org.usadellab.trimmomatic.fastq.FastqParser.parseOne(FastqParser.java:68)
	at org.usadellab.trimmomatic.fastq.FastqParser.parse(FastqParser.java:164)
	at org.usadellab.trimmomatic.fastq.FastqParser.parse(FastqParser.java:164)
	at org.usadellab.trimmomatic.TrimmomaticPE.process(TrimmomaticPE.java:265)
	at org.usadellab.trimmomatic.fastq.FastqParser.parse(FastqParser.java:164)
	at org.usadellab.trimmomatic.fastq.FastqParser.parse(FastqParser.java:164)
	at org.usadellab.trimmomatic.TrimmomaticPE.process(TrimmomaticPE.java:265)
	at org.usadellab.trimmomatic.fastq.FastqParser.parse(FastqParser.java:164)
	at org.usadellab.trimmomatic.fastq.FastqParser.parse(FastqParser.java:164)
	at org.usadellab.trimmomatic.TrimmomaticPE.process(TrimmomaticPE.java:265)
	at org.usadellab.trimmomatic.TrimmomaticPE.process(TrimmomaticPE.java:265)
	at org.usadellab.trimmomatic.TrimmomaticPE.run(TrimmomaticPE.java:555)
	at org.usadellab.trimmomatic.TrimmomaticPE.process(TrimmomaticPE.java:265)
	at org.usadellab.trimmomatic.TrimmomaticPE.process(TrimmomaticPE.java:265)
	at org.usadellab.trimmomatic.TrimmomaticPE.run(TrimmomaticPE.java:555)
	at org.usadellab.trimmomatic.TrimmomaticPE.process(TrimmomaticPE.java:265)
	at org.usadellab.trimmomatic.TrimmomaticPE.process(TrimmomaticPE.java:265)
	at org.usadellab.trimmomatic.TrimmomaticPE.run(TrimmomaticPE.java:555)
	at org.usadellab.trimmomatic.TrimmomaticPE.run(TrimmomaticPE.java:555)
	at org.usadellab.trimmomatic.Trimmomatic.main(Trimmomatic.java:80)
	at org.usadellab.trimmomatic.TrimmomaticPE.run(TrimmomaticPE.java:555)
	at org.usadellab.trimmomatic.TrimmomaticPE.run(TrimmomaticPE.java:555)
	at org.usadellab.trimmomatic.Trimmomatic.main(Trimmomatic.java:80)
	at org.usadellab.trimmomatic.TrimmomaticPE.run(TrimmomaticPE.java:555)
	at org.usadellab.trimmomatic.TrimmomaticPE.run(TrimmomaticPE.java:555)
	at org.usadellab.trimmomatic.Trimmomatic.main(Trimmomatic.java:80)
	at org.usadellab.trimmomatic.Trimmomatic.main(Trimmomatic.java:80)
	at org.usadellab.trimmomatic.Trimmomatic.main(Trimmomatic.java:80)
	at org.usadellab.trimmomatic.Trimmomatic.main(Trimmomatic.java:80)
	at org.usadellab.trimmomatic.Trimmomatic.main(Trimmomatic.java:80)
	at org.usadellab.trimmomatic.Trimmomatic.main(Trimmomatic.java:80)
cat: /opt/dex-seq/template/data_analysis/trimming/reads/F3D6S194_1.fastqcat: /opt/dex-seq/template/data_analysis/trimming/reads/F3D3S191_1.fastqcat: /opt/dex-seq/template/data_analysis/trimming/reads/F3D2S190_1.fastqcat: /opt/dex-seq/template/data_analysis/trimming/reads/F3D7S195_1.fastqcat: /opt/dex-seq/template/data_analysis/trimming/reads/F3D0S188_1.fastqcat: /opt/dex-seq/template/data_analysis/trimming/reads/F3D5S193_1.fastq: No such file or directory: No such file or directory: No such file or directory
: No such file or directory
: No such file or directory
: No such file or directory

2、关于mapping_file.txt文件映射关系，我是参考样例去配置的，但我还是有点懵，key、value可否讲一下具体含义，我看文档写的比较模棱两可，可否给出示例出来？
3、我的数据集是否有问题，或者说是选择数据库不对，导致出不来结果？

二. 项目环境

1、路径：/opt/dex-seq/template
2、raw_data文件命名
> F3D0S188_1.fastq.gz
F3D0S188_2.fastq.gz
F3D141S207_1.fastq.gz
F3D141S207_2.fastq.gz
F3D142S208_1.fastq.gz
F3D142S208_2.fastq.gz
F3D143S209_1.fastq.gz
F3D143S209_2.fastq.gz
F3D144S210_1.fastq.gz
F3D144S210_2.fastq.gz
F3D145S211_1.fastq.gz
F3D145S211_2.fastq.gz
F3D146S212_1.fastq.gz
F3D146S212_2.fastq.gz
F3D147S213_1.fastq.gz
F3D147S213_2.fastq.gz
F3D148S214_1.fastq.gz
F3D148S214_2.fastq.gz
F3D149S215_1.fastq.gz
F3D149S215_2.fastq.gz
F3D150S216_1.fastq.gz
F3D150S216_2.fastq.gz
F3D1S189_1.fastq.gz
F3D1S189_2.fastq.gz
F3D2S190_1.fastq.gz
F3D2S190_2.fastq.gz
F3D3S191_1.fastq.gz
F3D3S191_2.fastq.gz
F3D5S193_1.fastq.gz
F3D5S193_2.fastq.gz
F3D6S194_1.fastq.gz
F3D6S194_2.fastq.gz
F3D7S195_1.fastq.gz
F3D7S195_2.fastq.gz
F3D8S196_1.fastq.gz
F3D8S196_2.fastq.gz
F3D9S197_1.fastq.gz
F3D9S197_2.fastq.gz

3、 mapping_file.txt文件映射关系
>F3D0S188      F3D
F3D1S189      F3D
F3D2S190      F3D
F3D3S191      F3D
F3D5S193      F3D
F3D6S194      F3D
F3D7S195      F3D
F3D8S196      F3D
F3D9S197      F3D
F3D141S207    F3D
F3D142S208    F3D
F3D143S209    F3D
F3D144S210    F3D
F3D145S211    F3D
F3D146S212    F3D
F3D147S213    F3D
F3D148S214    F3D
F3D149S215    F3D
F3D150S216    F3D

4、执行命令：apptainer exec  -B  /opt/dex-seq/db /opt/dex-seq/apptainer/dix-seq-1.0.0.sif dix-seq /opt/dex-seq/template/metadata.txt validate
> metadata:
[1]: check parameter available:
parameter:[project_home]        [/opt/dex-seq/template/]        PASS
parameter:[project_id]  [data_analysis] PASS
parameter:[raw_data]    [/opt/dex-seq/template/raw_data]        PASS
parameter:[mapping_file]        [/opt/dex-seq/template/mapping_file.txt]        PASS
parameter:[cpus]        [80]    PASS
parameter:[parallel]    [8]     PASS
parameter:[threads]     [6]     PASS
parameter:[trim_param]  [2:30:10 SLIDINGWINDOW:4:15 LEADING:3 TRAILING:3 MINLEN:36]     PASS
parameter:[mergepairs_param]    [-fastq_minmergelen 250 -fastq_maxmergelen 500 -fastq_maxdiffs 10  -fastq_pctid 90  -fastq_minovlen 16   -fastq_trunctail 2 -fastq_minlen 64]   PASS
parameter:[search_pcr_param]    [-fwdprimer GTGCCAGCMGCCGCGG -revprimer CCGTCAATTCMTTTRAGTTT -minamp 250 -maxamp 480]   PASS
parameter:[db]  [/opt/dex-seq/db]       PASS
parameter:[maxee]       [1]     PASS
parameter:[unoise3_param]       [-minsize 8]    PASS
parameter:[reference]   [rdp_16s_v18_sp]        PASS
parameter:[sintax_cutoff]       [0.8]   PASS
parameter:[filter]      [NONE]  PASS
parameter:[tree]        [distmx]        PASS
parameter:[rarefaction] [500:500:50000] PASS
parameter:[text]        [T]     PASS
parameter:[pathway]     [F]     PASS
parameter:[vegdist]     [bray]  PASS
[2]: check path available:
parameter:[project_home]        path:[/opt/dex-seq/template/]   PASS
parameter:[raw_data]    path:[/opt/dex-seq/template/raw_data]   PASS
parameter:[mapping_file]        path:[/opt/dex-seq/template/mapping_file.txt]   PASS
[3]: check reads available:
F3D0S188:       /opt/dex-seq/template/raw_data/F3D0S188_1.fastq.gz,/opt/dex-seq/template/raw_data/F3D0S188_2.fastq.gz   PASS
F3D1S189:       /opt/dex-seq/template/raw_data/F3D1S189_1.fastq.gz,/opt/dex-seq/template/raw_data/F3D1S189_2.fastq.gz   PASS
F3D2S190:       /opt/dex-seq/template/raw_data/F3D2S190_1.fastq.gz,/opt/dex-seq/template/raw_data/F3D2S190_2.fastq.gz   PASS
F3D3S191:       /opt/dex-seq/template/raw_data/F3D3S191_1.fastq.gz,/opt/dex-seq/template/raw_data/F3D3S191_2.fastq.gz   PASS
F3D5S193:       /opt/dex-seq/template/raw_data/F3D5S193_1.fastq.gz,/opt/dex-seq/template/raw_data/F3D5S193_2.fastq.gz   PASS
F3D6S194:       /opt/dex-seq/template/raw_data/F3D6S194_1.fastq.gz,/opt/dex-seq/template/raw_data/F3D6S194_2.fastq.gz   PASS
F3D7S195:       /opt/dex-seq/template/raw_data/F3D7S195_1.fastq.gz,/opt/dex-seq/template/raw_data/F3D7S195_2.fastq.gz   PASS
F3D8S196:       /opt/dex-seq/template/raw_data/F3D8S196_1.fastq.gz,/opt/dex-seq/template/raw_data/F3D8S196_2.fastq.gz   PASS
F3D9S197:       /opt/dex-seq/template/raw_data/F3D9S197_1.fastq.gz,/opt/dex-seq/template/raw_data/F3D9S197_2.fastq.gz   PASS
F3D141S207:     /opt/dex-seq/template/raw_data/F3D141S207_1.fastq.gz,/opt/dex-seq/template/raw_data/F3D141S207_2.fastq.gz       PASS
F3D142S208:     /opt/dex-seq/template/raw_data/F3D142S208_1.fastq.gz,/opt/dex-seq/template/raw_data/F3D142S208_2.fastq.gz       PASS
F3D143S209:     /opt/dex-seq/template/raw_data/F3D143S209_1.fastq.gz,/opt/dex-seq/template/raw_data/F3D143S209_2.fastq.gz       PASS
F3D144S210:     /opt/dex-seq/template/raw_data/F3D144S210_1.fastq.gz,/opt/dex-seq/template/raw_data/F3D144S210_2.fastq.gz       PASS
F3D145S211:     /opt/dex-seq/template/raw_data/F3D145S211_1.fastq.gz,/opt/dex-seq/template/raw_data/F3D145S211_2.fastq.gz       PASS
F3D146S212:     /opt/dex-seq/template/raw_data/F3D146S212_1.fastq.gz,/opt/dex-seq/template/raw_data/F3D146S212_2.fastq.gz       PASS
F3D147S213:     /opt/dex-seq/template/raw_data/F3D147S213_1.fastq.gz,/opt/dex-seq/template/raw_data/F3D147S213_2.fastq.gz       PASS
F3D148S214:     /opt/dex-seq/template/raw_data/F3D148S214_1.fastq.gz,/opt/dex-seq/template/raw_data/F3D148S214_2.fastq.gz       PASS
F3D149S215:     /opt/dex-seq/template/raw_data/F3D149S215_1.fastq.gz,/opt/dex-seq/template/raw_data/F3D149S215_2.fastq.gz       PASS
F3D150S216:     /opt/dex-seq/template/raw_data/F3D150S216_1.fastq.gz,/opt/dex-seq/template/raw_data/F3D150S216_2.fastq.gz       PASS

三、相关日志：

[logs.zip](https://github.com/user-attachments/files/21249349/logs.zip)


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大佬们求助！我换了个元数据集，运行失败问题 #2

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大佬们求助！我换了个元数据集，运行失败问题 #2

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