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Description
I successfully ran Guppy for basecalling, and the process completed without any errors. However, I noticed that no FASTQ files were generated in the output directory.
Here is the output from the run:
ONT Guppy duplex basecalling software version 6.0.6+8a98bbc
config file: /input/model.cfg
model file: /input/model.jsn
input path: /input/demo
save path: output/call_res
chunk size: 2000
chunks per runner: 256
minimum qscore: 0
records per file: 5000
num basecallers: 4
gpu device: cuda:1
kernel path:
runners per device: 12
Found 2 fast5 files to process.
Init time: 1676 ms
0% 10 20 30 40 50 60 70 80 90 100%
|----|----|----|----|----|----|----|----|----|----|
***************************************************
Caller time: 2102 ms, Samples called: 465044, samples/s: 221239
Finishing up any open output files.
Failed to send ping to https://ping-dev.oxfordnanoportal.com/basecall: Failed to connect to ping-dev.oxfordnanoportal.com port 443: No route to host: Couldn't connect to server
Basecalling completed successfully.
Here is the generated sequencing_summary.txt:
filename read_id duplex_pair_read_id run_id batch_id channel mux start_time duration num_events passes_filtering template_start num_events_template template_duration sequence_length_template mean_qscore_template strand_score_template median_template mad_template scaling_median_template scaling_mad_template
temps_labd.fast5 read_channel1_cp0 read_channel1_cp1 PC28 0 1 1 42.594400 78.329800 78329 TRUE 42.620400 78303 78.303800 5060 6.228380 0 0.572205 0.076294 0.572205 0.076294
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