diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index b6419042de01..e12c961a3c9b 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -389,8 +389,8 @@ jobs:
             PATH: pyopenssl
           - DOWNSTREAM: twisted
             REPO: twisted/twisted
-            # Latest commit on the twisted trunk branch, as of Aug 14, 2026.
-            REF: 4ffe6a4a4683f670b3615f0800d1111d8c342891
+            # Latest commit on the twisted trunk branch, as of Aug 16, 2026.
+            REF: 0e00a4f8ba2e33a1a257ed316d3ace53e94e1c50
             PATH: twisted
           - DOWNSTREAM: aws-encryption-sdk-python
             REPO: awslabs/aws-encryption-sdk-python