Hi, we have been using your great tool for several years and saved as a lot of disc space! However, recently we have encountered and error that appears during DSRC encoding. Algorithm occasionally skips a number of reads at seemingly random position and then continues. The resulting file contain artifacts like this:
@L183:321:CAFVJANXX:6:2213:18462:88964 3:N:0:0
TATAAATGGATTCTCTTTGTCCATGATCACAAAATAAGAAT@L183:321:CAFVJANXX:6:2213:5699:93216 3:N:0:0
Renaming the reads solves the problem.
Do you happen to know what may cause such issues?
The problems were encountered with this public dataset: https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-10175/
In particular, the problem can be reproduced with this file: http://ftp.sra.ebi.ac.uk/vol1/run/ERR539/ERR5396174/AML_low_input_AAAACT_r2.fq.gz
Many thanks for any information in advance,
Best,
Ievgen
Hi, we have been using your great tool for several years and saved as a lot of disc space! However, recently we have encountered and error that appears during DSRC encoding. Algorithm occasionally skips a number of reads at seemingly random position and then continues. The resulting file contain artifacts like this:
Renaming the reads solves the problem.
Do you happen to know what may cause such issues?
The problems were encountered with this public dataset: https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-10175/
In particular, the problem can be reproduced with this file: http://ftp.sra.ebi.ac.uk/vol1/run/ERR539/ERR5396174/AML_low_input_AAAACT_r2.fq.gz
Many thanks for any information in advance,
Best,
Ievgen