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i want to exclude N >1000 reads for RNA bam files, for example, the reads "9ae8a1cd-1a71-40d1-ab06-f1567ec79f9a" contained 1593N, it should be filtered. However , this software not work when I used this code "variant GW004.ONT.RNA.sorted.filter.chr7.bam --max-nbases 1000 -o GW004.ONT.RNA.sorted.filter.chr7.N10k.bam -b -t100"
This why? Thanks~
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