[Study]: Gut–ovary axis and multiomic insights into PCOS in a DHEA-induced rat model

[Buraah]

The Digital Object Identifier (DOI)

10.1038/s41598-025-30862-0

Code of Conduct Agreement

• I agree to follow this project's Code of Conduct

[github-actions]

Title

Gut–ovary axis and multiomic insights into PCOS in a DHEA-induced rat model

DOI

https://doi.org/10.1038/s41598-025-30862-0

PMID

41360922

Author

Zhi Chen

Journal

Scientific Reports

Year

2025

👋 Thanks for submitting!

[EjiroOS]

Hello @SvetlanaUP, Please i would like to curate this paper as my second contribution, Thank you

[EjiroOS]

Hello @SvetlanaUP
I have completed the curation for this paper.
BugSigDB study page: https://bugsigdb.org/41360922
Paper: Gut–ovary axis and multiomic insights into PCOS in a DHEA-induced rat model. Zhi Chen et al., Scientific Reports (2025). PMID: 41360922.
1 experiment created: Gut microbiota in DHEA-induced PCOS rats vs. sesame oil-treated controls. Host: Rattus norvegicus, Body site: Feces, 16S rRNA V3-V4, Illumina, n=14 PCOS vs n=11 Control.
2 signatures created:

Signature 1 — Increased in PCOS: Allobaculum, Corynebacterium, Jeotgalicoccus (Fig. 2D; Fig. 2E)
Signature 2 — Decreased in PCOS: Bacillus, Staphylococcus (Fig. 2D)

This curation is ready for review. Thank you!

[TheTosin]

Good afternoon @SvetlanaUP

I’d like to review this study, thank you

[TheTosin]

Hi @EjiroOS, good job done here curating your first study! I have some observations below:

• Figure 7 is curatable. A spearman correlation analysis with 4 different comparisons containing significant results. One of them is low level of E2 (group 0) vs High level of E2 (group 1).
• Begin the group 1 name with a capital letter “Polycystic ovary syndrome rats (PCOS).
• Curate a seperate experiment with the statistical test: LEfSe for Figure 2E result.

Experiment 1:

• The data transformation for Mann Whitney is “relative abundance”. MHT is No, also remove the LDA score and leave it empty as it applies only to LEfSe results.
• Alpha diversity: Shannon should also be recorded as unchanged as it is was stated to be non-significant (NS) from figure 2A.
• Source should be “Figure 2D” only. Kindly remove Fig. 2E as it contains details from another test entirely.
• Remove “and Linear Discriminant Analysis” from signature 1 description.
• Add Prevotellaceae NK3B31 group and A2 to Signature 1. Figure 2D states the p value of both taxa to be less than 0.05 hence it’s significant and should be curated as well.
• In signature 2: Add Roseburia, Blautia and Bacilli

I'll be looking forward to reviewing the corrected curation🌷.

Item	Description	max	points
1	All elements marked "Needs review" (none "Incomplete")	1	1
2	Correct study design	1	1
3	Entered all relevant experiments and no irrelevant experiments	1	0.5
4	Body site correctly identified (i.e. does not include multiple sites)	1	1
5	Condition entered according to contrast (correct EFO ontology)	1	1
6	Contrast groups correctly identified	1	1
7	Groups correctly labeled as 1 and 0 (1=cases, 0=controls)	1	1
8	Antibiotic exclusion correctly identified	1	1
9	Correctly identified sequencing details	2	2
10	Identified correct statistical test	1	1
11	Identified MHT correction	1	0
12	Correctly recorded matched on factors	1	1
13	Entered correct number of statistical tests per experiment	1	0.5
14	All diversity measures identified	1	0.5
15	Diversity results correctly entered as increased/decreased/unchanged	1	1
16	All signature sources correctly identified (-1 for each error)	2	1
17	Abundance direction correctly selected	1	1
18	Members of Signatures identified correctly	2	1
19	Correct use of NCBI taxonomy	2	2
	TOTAL	23	18.5

[EjiroOS]

Hello @TheTosin thank you very much for your feedback. Here is the updated curation putting into consideration your feedback: https://bugsigdb.org/41360922
For experiment 2, I experienced some challenges adding 2 extra signatures(signature 2 and 3, respectively) that I couldn't delete, here: https://bugsigdb.org/Special:FormEdit/Signature/41360922/Experiment_2/Signature_2?reload=1&returnto=41360922 https://bugsigdb.org/Special:FormEdit/Signature/41360922/Experiment_2/Signature_3?reload=1&returnto=41360922
I would appreciate feedback. Thank you

[SvetlanaUP]

https://bugsigdb.org/41360922/Experiment_2/Signature_2
https://bugsigdb.org/41360922/Experiment_2/Signature_3
deleted.

[EjiroOS]

Thank you @SvetlanaUP
Hello @SvetlanaUP and @TheTosin , I have made all the corrections based on the feedback provided and updated my curation.
BugSigDB study page: https://bugsigdb.org/41360922
Summary of corrections made:

Group 1 name updated to "Polycystic ovary syndrome rats (PCOS)"
Data transformation set to "relative abundance" for Experiment 1
MHT correction set to "No" for Experiment 1
LDA Score removed from Experiment 1
Shannon alpha diversity added as "unchanged" in Experiment 1
Signature 1 source updated to "Fig. 2D" only
"Linear Discriminant Analysis" removed from Signature 1 description
Prevotellaceae NK3B31 group and A2 added to Signature 1
Roseburia, Blautia and Bacilli added to Signature 2

New experiments added:

Experiment 2: LEfSe analysis (Fig. 2E) — Signature: Allobaculum, Corynebacterium, Jeotgalicoccus (increased in PCOS)
Experiment 3: Spearman correlation — E2 — Signature: Bacillus, Roseburia (decreased in High E2)
Experiment 4: Spearman correlation — LH — Signature: Corynebacterium, Coriobacteriaceae UCG-002 (decreased in High LH)
Experiment 5: Spearman correlation — FSH — Signature: Adlercreutzia (decreased in High FSH)
Experiment 6: Spearman correlation — T — Signature 1: Bacillus, Jeotgalicoccus, unclassified_c__Bacilli (increased in High T); Signature 2: A2 (decreased in High T)

This curation is now ready for review. Thank you for your patience and detailed feedback!