non-normalised histogram comparison?

[celine304]

Hi Wouter,

I'm trying to compare sequencing data from two different sequencing platforms (Pacbio sequel and revio) on the same graph but I can only get a graph where both have the same surface area but the read number for one is much higher than the other. Is there a way to not normalise the data like this as it does for nanoplot but show them in the same graph?

Thanks,
Celine

[wdecoster]

Hmm I would expect it not to normalize the dataset... remarkable. Let me look into that, but I will only have time for that after my holidays.

[celine304]

Thanks Wouter, enjoy your holidays :)

[wdecoster]

Hrm it looks okay in a toy test with 50 and 300 reads:

In your plot, how much higher should the read number be for revio? There is at least a large difference in the ~5kb range...

[celine304]

The sequel run had 2.2 million reads compared to 8.6 million for the revio