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2 changes: 2 additions & 0 deletions WIP/voet-pmdbs-sn-atacseq-10x/DOI/10.5281_zenodo.18988729
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116 changes: 116 additions & 0 deletions WIP/voet-pmdbs-sn-atacseq-10x/DOI/beta-deposition.json
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"description": "<p>This Zenodo deposit contains a publicly available description of the Dataset:</p>\n<p><strong>Title:</strong> \"Single nuclei ATAC sequencing of postmortem cingulate cortex,midbrain and motor cortex of healthy donors and Parkinson's disease patients \u2013 10x snATAC-seq.\".</p>\n<p><strong>Description:</strong> This dataset consists of raw sequencing snATAC-seq data (10x Genomics Chromium Next GEM Single Cell ATAC v2). The data is part of an overall set of samples derived from postmortem midbrain (n=140), cingulate cortex (n=190) and motor cortex (n=4) of healthy donors (n=114), patients with Parkinson's disease (n=75) or patients with other neurological disorder (n=1). The protocol followed to isolate nuclei from postmortem brain samples and to prepare sequencing libraries can be found here: https://www.protocols.io/view/nuclei-isolation-from-brain-tissue-for-single-cell-14egnrb7ql5d/v1. To increase throughput and to decrease batch effects, several donors have been pooled together into a single sequencing library. To computationally demultiplex the nuclei to their corresponding donors, cellsnp-lite (version commit: aad18644adcde853c313362a856a24245c9b91f7) followed by vireo (https://github.com/single-cell-genetics/vireo/pull/108 ) has been used. The population VCF with the donor genotypes derived from whole genome sequencing data has been used to assign nuclei back to their donors.</p>\n\n\n<blockquote>\n<p>This dataset is made available to researchers via the ASAP CRN Cloud: <a href=\"https://cloud.parkinsonsroadmap.org\">cloud.parkinsonsroadmap.org</a>. Instructions for how to request access can be found in the <a href=\"https://storage.googleapis.com/asap-public-assets/wayfinding/ASAP-CRN-Cloud-User-Manual.pdf\">User Manual</a>.</p>\n<p>This research was funded by the Aligning Science Across Parkinson's Collaborative Research Network (ASAP CRN), through the Michael J. Fox Foundation for Parkinson's Research (MJFF).</p>\n<p>This Zenodo deposit was created by the ASAP CRN Cloud staff on behalf of the dataset authors. It provides a citable reference for a CRN Cloud Dataset</p>\n</blockquote>",
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1 change: 1 addition & 0 deletions WIP/voet-pmdbs-sn-atacseq-10x/DOI/dataset.doi
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56 changes: 56 additions & 0 deletions WIP/voet-pmdbs-sn-atacseq-10x/DOI/project.json
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{
"project_name": "Understanding inherited and acquired genetic variation in Parkinson's disease through single-cell multi-omics analyses: a unique data resource",
"project_description": "The functional roles of inherited and acquired genetic variation in the pathogenesis of Parkinson's disease (PD) remain largely unknown. Here, we will first study how germline genetic variants at PD-risk loci, which were identified by genome-wide association study (GWAS), perturb the expression of genes in specific cell (sub)populations of the brain and gut. To this aim, we will apply single-cell gene-expression and open-chromatin quantitative trait locus (QTL) analyses, enabling identification of PD-relevant genes and cell (sub)types. We will deliver the mechanisms of PD-candidate gene expression (dys)regulation in the normal condition, with ageing and in PD, as well as a unique single-cell multi-omic resource for the community. Second, we will study the nature and role of somatic mutations in brain and gut cells in PD- etiopathology. Finally, we will characterize biochemical and phenotypic effects of loss- or gain-of-function QTLs and somatic mutations of candidate genes in in vitro and in vivo model systems, including their impact on the neuro-immune axis.",
"dataset_title": "Single nuclei ATAC sequencing of postmortem cingulate cortex,midbrain and motor cortex of healthy donors and Parkinson's disease patients \u2013 10x snATAC-seq.",
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"creators": [
{
"name": "Pan\u010d\u00edkov\u00e1, Alexandra",
"affiliation": "Laboratory of Computational Biology, VIB Center for AI & Computational Biology (VIB.AI), Leuven, Belgium\nVIB-KU Leuven Center for Brain & Disease Research, Leuven, Belgium.\nDepartment of Human Genetics, KU Leuven, Leuven, Belgium\nLaboratory of Integrative Cancer Genomics, VIB-KU Leuven Center for Cancer Biology, Leuven, Belgium\nDepartment of Oncology, KU Leuven, Leuven, Belgium",
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},
{
"name": "Theunis, Koen",
"affiliation": "Laboratory of Computational Biology, VIB Center for AI & Computational Biology (VIB.AI), Leuven, Belgium\nVIB-KU Leuven Center for Brain & Disease Research, Leuven, Belgium.\nDepartment of Human Genetics, KU Leuven, Leuven, Belgium",
"orcid": "0000-0002-0699-6676"
},
{
"name": "Hulselmans, Gert",
"affiliation": "Laboratory of Computational Biology, VIB Center for AI & Computational Biology (VIB.AI), Leuven, Belgium\nVIB-KU Leuven Center for Brain & Disease Research, Leuven, Belgium.\nDepartment of Human Genetics, KU Leuven, Leuven, Belgium",
"orcid": "0000-0003-2205-1899"
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{
"name": "Sigalova, Olga",
"affiliation": "Laboratory of Computational Biology, VIB Center for AI & Computational Biology (VIB.AI), Leuven, Belgium\nVIB-KU Leuven Center for Brain & Disease Research, Leuven, Belgium.\nDepartment of Human Genetics, KU Leuven, Leuven, Belgium",
"orcid": "0000-0001-8598-1079"
},
{
"name": "De Man, Julie",
"affiliation": "Laboratory of Computational Biology, VIB Center for AI & Computational Biology (VIB.AI), Leuven, Belgium\nVIB-KU Leuven Center for Brain & Disease Research, Leuven, Belgium.\nDepartment of Human Genetics, KU Leuven, Leuven, Belgium",
"orcid": "0009-0003-7208-8961"
},
{
"name": "Voet, Thierry",
"affiliation": "Department of Human Genetics, KU Leuven, Leuven, Belgium\nKU Leuven Institute for Single Cell Omics (LISCO), University of Leuven, KU Leuven, Leuven, Belgium",
"orcid": "0000-0003-1204-9963"
},
{
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"affiliation": "Laboratory of Computational Biology, VIB Center for AI & Computational Biology (VIB.AI), Leuven, Belgium\nVIB-KU Leuven Center for Brain & Disease Research, Leuven, Belgium.\nDepartment of Human Genetics, KU Leuven, Leuven, Belgium",
"orcid": "0000-0002-8006-0315"
}
],
"publication_date": "30-09-2025",
"version": "1.0",
"title": "Single nuclei ATAC sequencing of postmortem cingulate cortex,midbrain and motor cortex of healthy donors and Parkinson's disease patients \u2013 10x snATAC-seq.",
"ASAP_lab_name": "Aerts Lab",
"PI_full_name": "Stein Aerts",
"PI_email": "stein.aerts@kuleuven.be",
"submitter_name": "Gert Hulselmans",
"submitter_email": "gert.hulselmans@kuleuven.be",
"publication_DOI": "NA",
"grant_ids": [
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],
"team_name": "Voet"
}
1 change: 1 addition & 0 deletions WIP/voet-pmdbs-sn-atacseq-10x/DOI/version.doi
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