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September 20, 2025 23:50
…tes_to_b2f_report
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Updates to Bases2Fastq Report. Changes include:
Generation of a complete report for Project level samples.
Sequencing Run QC Metrics Table
Polonies should be a count, so it shows 9,067,812 Polonies vs 9.1 Mb
Add cycles per read
Add # of “Reads eliminated” from adapter trimming (found in RunStats)
Add # of “Mean read length” from adapter trimming (found in RunStats)
If possible, Add # of “Min read length” from adapter trimming (found in run manifest)
If possible, “Trim type” from adapter trimming (found in run manifest)
Add Indices table (All indices, Top Unassigned Sequences) show 30 sequences by default
Add a histogram for sample assignment - limit to 1000 samples
Sequencing Run Yield
on Yield change x axis to “Yield”
Run Base Quality Histogram
Fix typo (QualitierQuality per Read)
Add x axis label “Q score”
Quality Metrics by cycle
Add “Read 1 and Read 2 are separated by a red dashed line”
Sample QC Metrics Table
Show mismatch
Per Cycle Base Content
Change description to “Base composition per sample per cycle. Read 1 and Read 2 are separated by a red dashed line”
Change Y axis to “Percentage of total reads”
Per cycle Adapter Content
Add to description: “Read 1 and Read 2 are separated by a red dashed line”
Per Sample GC Histogram
change X axis to “GC Content (%)“
change Y axis to “Percentage of reads that are GC (%)”