Skip to content
Open
Show file tree
Hide file tree
Changes from all commits
Commits
Show all changes
47 commits
Select commit Hold shift + click to select a range
fc8c309
overhaul 1
Aug 19, 2020
c5d01da
overhaul 1
Aug 19, 2020
5000122
overhaul 1
Aug 19, 2020
8e83e98
overhaul 1
Aug 19, 2020
4d4ce66
overhaul 1
Aug 20, 2020
5a137a4
overhaul 1
Aug 20, 2020
28c0944
Merge pull request #3 from leahkemp/overhaul_1
sirselim Aug 20, 2020
078290d
fixes
Aug 20, 2020
8dbabc5
fixes
Aug 20, 2020
16fa9e4
fixes
Aug 20, 2020
1771cb4
fixes
Aug 20, 2020
b91931d
fixes
Aug 24, 2020
df1fb72
fixes
Aug 25, 2020
58a6122
fixes
Aug 31, 2020
1ce29c2
Add pipeline assumtion
leahkemp Aug 31, 2020
e64e13a
Add requirement to label pedigree files with proband name
leahkemp Sep 1, 2020
793e019
Update README
leahkemp Sep 1, 2020
4d044eb
Update README
leahkemp Sep 1, 2020
5391633
Update workflow.rst
leahkemp Sep 1, 2020
b5af23a
Update workflow.rst
leahkemp Sep 1, 2020
950e18c
Allow user to set the adapter trimming settings
Sep 4, 2020
747246a
Merge pull request #5 from ESR-NZ/configure_adapters
leahkemp Sep 4, 2020
cd42eec
Allow user to set the adapter trimming settings
leahkemp Sep 4, 2020
0f75720
Fix multiqc wrapper + slurm issue
Sep 6, 2020
53aa37d
Merge pull request #6 from leahkemp/multiqc_slurm_patch
leahkemp Sep 6, 2020
9c7a816
Merge cpu and gpu pipelines
Sep 8, 2020
c2e6ece
Remove parabricks rules that don't run on GPU's
Sep 9, 2020
b559b7a
Merge pull request #7 from leahkemp/merge_cpu_gpu
leahkemp Sep 9, 2020
9f8e7d2
Add HPC run scripts
Sep 9, 2020
92fbd9d
Update README.md
leahkemp Sep 9, 2020
0c22dfb
Add optional fastq trimming
Sep 9, 2020
efb2c59
Merge pull request #8 from leahkemp/optional_trimming
leahkemp Sep 9, 2020
98542c4
Add back one of the gpu rule because of gatk inconsistancies between …
Sep 9, 2020
0baa7e2
Integrate trimming reports into multiqc report
Sep 10, 2020
cff5e09
Merge pull request #9 from leahkemp/extend_multiqc_report
leahkemp Sep 10, 2020
24b1017
Update README.md
leahkemp Sep 10, 2020
cfb9dbe
Fix
leahkemp Sep 21, 2020
1b94574
Update README.md
leahkemp Sep 29, 2020
4e10ddc
Allow per rule/sample resource configuration + other fixes
Oct 6, 2020
42242df
Merge pull request #10 from leahkemp/manage_resources
leahkemp Oct 7, 2020
95590f7
Update pbrun_fq2bam.smk
leahkemp Oct 8, 2020
6e62500
Fix
leahkemp Oct 9, 2020
2fdc57b
Remove pbrun_genotypegvcf rule - doesn't actually use GPUs
Oct 9, 2020
a4dc43d
Make gatk conda env compatible with parabricks gatk version
leahkemp Oct 12, 2020
df56d39
Patch_1 (#13)
leahkemp Nov 12, 2020
9b5c8a0
Dev (#23)
leahkemp Jan 7, 2022
1a6f3d0
Release v2.1.0 (#24)
leahkemp Apr 19, 2022
File filter

Filter by extension

Filter by extension

Conversations
Failed to load comments.
Loading
Jump to
Jump to file
Failed to load files.
Loading
Diff view
Diff view
8 changes: 0 additions & 8 deletions .gitignore

This file was deleted.

352 changes: 69 additions & 283 deletions README.md

Large diffs are not rendered by default.

59 changes: 0 additions & 59 deletions Snakefile

This file was deleted.

54 changes: 0 additions & 54 deletions cluster.json

This file was deleted.

File renamed without changes.
8 changes: 8 additions & 0 deletions config/cluster.json
Original file line number Diff line number Diff line change
@@ -0,0 +1,8 @@
{
"__default__" :
{
"account" : "",
"partition" : "",
"output" : "logs/slurm-%j_{rule}.out"
}
}
60 changes: 60 additions & 0 deletions config/config.yaml
Original file line number Diff line number Diff line change
@@ -0,0 +1,60 @@
##############################
###### Overall workflow ######
##############################

# Type of input data (either 'Single' or 'Cohort')
DATA: ""

# Should the pipeline be GPU accelerated where possible? (either 'Yes' or 'No')
GPU_ACCELERATED: ""

# File path to the reference genome (.fasta)
REFGENOME: ""

# File path to dbSNP database
dbSNP: ""

# Temporary file directory
TEMPDIR: ""

# Whole exome sequence settings (leave blank if analysing other data such as whole genome sequence data)
WES:
# File path to the exome capture regions over which to operate
INTERVALS: ""
# Padding (in bp) to add to each region
PADDING: ""

##############################
##### Pipeline resources #####
##############################

# Number of threads to use per rule/sample for multithreaded rules, multithreading will significantly speed up these rules (diminishing speed gains beyond 8 threads)
THREADS:

# Maximum memory usage per rule/sample (eg. '40g' for 40 gigabytes, this should suffice for exomes)
MAXMEMORY: ""

# Maximum number of GPU's to be used per rule/sample for gpu-accelerated runs (eg `1` for 1 GPU)
GPU:

##############################
########## Trimming ##########
##############################

# Whether or not to trim the raw fastq reads (either 'Yes' or 'No')
TRIM: ""

# If trimming, choose the adapter sequence to be trimmed (eg. `--illumina`, `--nextera` or `--small_rna`) or pass adapter sequences to the `-a` and `-a2` flags
TRIMMING:
ADAPTERS: ""

##############################
##### Base recalibration #####
##############################

# List of resources to used for base recalibration
RECALIBRATION:
RESOURCES:
-
-
-
30 changes: 0 additions & 30 deletions config_examples/GRCh37_cohort_WES_config_template.yaml

This file was deleted.

30 changes: 0 additions & 30 deletions config_examples/GRCh37_cohort_WGS_config_template.yaml

This file was deleted.

30 changes: 0 additions & 30 deletions config_examples/GRCh37_single_WES_config_template.yaml

This file was deleted.

30 changes: 0 additions & 30 deletions config_examples/GRCh37_single_WGS_config_template.yaml

This file was deleted.

29 changes: 0 additions & 29 deletions config_examples/GRCh38_cohort_WES_config_template.yaml

This file was deleted.

29 changes: 0 additions & 29 deletions config_examples/GRCh38_cohort_WGS_config_template.yaml

This file was deleted.

Loading