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% This Readme file describes version and operation instructions for % Two Photon and Behavioral analysis tools. % % User Manual : % .\Doc\TwoPhotonAnalysis UserGuide.docx % % To start: % run TPA_MainGUI - gui mode % % Acknowledgements: % Special thanks for debugging - Jackie Schiller, Maria Lavzin, Liora Garion, Adam Hantman % Code contributions from: % 1. Piotr Dollar toolbox for automatic classification of events. % 2. Image registration - Janelia code originally written by Sun Wenzhi, 8/28/2012 for Image Box SW. % This code uses algorithms developed by Ann M. Kowalczyk and James R. Fienup in paper % J.R. Fienup and A.M. Kowalczyk, "Phase retrieval for a complex-valued object by using a low-resolution image," J. Opt. Soc. Am. A 7, 450-458, (1990). % 3. For the rest you can blame me Uri Dubin. % % To Do List: % 1. %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% % Ver Date Who What %================================================== % 29.04 19.03.19 UD Gal event import fix % 29.03 19.03.19 UD Multiple channels multiple slices support. Fixing bug in Event alignment. % 29.02 10.10.18 UD Fix computer name change in Video Labeler. % 29.01 26.09.18 UD Can load new system files. % 28.28 14.08.18 UD Video Labeler - fixes. % 28.27 25.07.18 UD Video Labeler - filtering of ROIs. % 28.26 08.07.18 UD Video Labeler updates. Fixes for Shahar % 28.25 04.07.18 UD Hadas Averaging is merged back from 2710. % 28.24 27.06.18 UD VideoLabeler updates - training. % 28.23 26.06.18 UD VideoLabeler updates. % 28.22 13.06.18 UD Export to excel for Fadi. % 28.21 06.06.18 UD Testing behavioral labeler again. Adding snippets. % 28.20 28.05.18 UD Gal is coding. % 28.19 24.05.18 UD Overlay of snippets. Merging with Gal. % 28.18 09.05.18 UD Import from Labeler. Adding Scripts for Jackie % 28.17 29.04.18 UD Export data from Labeler and fixes for Faddy % 28.16 11.04.18 UD Trajectory tool improvement % 28.15 01.04.18 UD Ethogram export % 28.14 29.03.18 UD ElectroPhys for certain simple file and Averaging for Shahar. % 28.13 19.03.18 UD ElectroPhys for Fadi. % 28.12 08.03.18 UD Improving Behavioral analysis tool. Fadi behavioral offset by 20 frames. % 28.11 04.03.18 UD Updates in technion % 28.10 27.02.18 UD Running on bigger dataset % 28.09 19.02.18 UD Integrating Matlab GUI for ROI labeling % 28.08 09.02.18 UD DNN Heatmaps. Fixing selection for Fadi. Trajectory extraction. % 28.07 30.01.18 UD Gals code integration % 28.06 22.01.18 UD New dF/F for inhibition - multiple trials. Gal's code for detection of neurons. % 28.05 19.01.18 UD New dF/F for inhibition % 28.04 15.01.18 UD Fixing Excel export with names of trials and original brightness. % 28.03 07.01.18 UD Fixing bugs in Import for Shahar. % 28.02 03.01.18 UD Fixing Multitrial bugs for Fadi. Import timestamp mat files for Shahar. % 28.01 28.12.17 UD Fadi two channel system import. % 27.14 26.12.17 UD Fixing export to excel. % 27.13 17.12.17 UD Event alignment threshold is lowered. % 27.12 04.12.17 UD Working on trajectories. % 27.11 22.11.17 UD Adding ROI tracking tool. Figures are left out. % 27.10 08.11.17 UD Looking on averaged df/f and behavior traces % 27.09 07.11.17 UD Analysis of averages % 27.08 31.10.17 UD Fixing alignment tool - save image as tif. % 27.07 29.10.17 UD Fixing alignment tool. % 27.06 24.10.17 UD Adding alignment tool for ROIs. % 27.05 22.10.17 UD Trying to improve trajectories. % 27.04 22.10.17 UD Fixes for Pearson and cell selection. % 27.03 16.10.17 UD Developing cell detection using RCNN. Skip 2702 - at home % 27.01 08.10.17 UD Cell detection RCNN % 26.06 16.07.17 UD Improving clustering % 26.05 12.07.17 UD Working with Jackie - improving trajectories % 26.04 02.07.17 UD Browsing tool in Multitrial improved % 26.03 19.06.17 UD Segmentation auto tools % 26.02 13.06.17 UD MultuTrial Explorer - show cell 2D position. % 26.01 06.06.17 UD SVD representation mutlitrial. % 26.00 01.06.17 UD Import from Janelia image stack. % 25.14 25.05.17 UD Adding noise of 10 to dFF in Image tool. % 25.13 23.05.17 UD Adding tool Z stack fix. % 25.12 18.05.17 UD Fixing bug in Event S+1. MaxTrialNum. Fixing merge bug % 25.11 10.05.17 UD Event S+1. Moving some MultiTrialEvent files to MultiTrialEventManager. % 25.10 04.05.17 UD Alignment tool for Z stacks. Fadi spike detector for all. % 25.09 27.04.17 UD Detection of ROIs. Do not remove old events. % 25.08 19.04.17 UD Memory of behavioral analysis improvement. Adding Manual event detect. % 25.07 09.04.17 UD JPCA. TBD. % 25.06 05.04.17 UD Fixing behavioral show. % 25.05 03.04.17 UD Adding show fluorescence. Fadi - removing trend to detect long events. Filter MaxRespWidth 20. % Faster behavioral data load % 25.04 19.03.17 UD Events Behavior debug. % 25.03 14.03.17 UD Events Behavior. % 25.02 07.03.17 UD Merging with 24.17. Working on events construction. % 25.01 06.02.17 UD Behavior Analysis as events. Jaaba merge option. % 24.12 22.11.16 UD bug fix from Shahar. % 24.11 15.11.16 UD Updates from Jackie. % 24.10 12.11.16 UD Rechecking Behavioral Events in Multi Experiment processing. MinFluorescentLevel = 20 % 24.09 08.11.16 UD Adding ROI size rescaling and Multi Experiment processing % 24.08 01.11.16 UD Fixing Bug with Time rescaling % 24.07 26.10.16 UD Fixing Electro Phys import for Fadi % 24.06 16.10.16 UD Support Bahavior 200Hz and Image Rate 30 - not an even number. % 24.05 12.09.16 UD Adding registration. % 24.04 06.09.16 UD Debug in the Lab. Fixing counter. % 24.03 02.09.16 UD Debugging data manager in memory and session % 24.02 23.08.16 UD Adding data manager in memory and session % 23.21 23.08.16 UD Fixing bugs for Fadi flow % 23.20 23.08.16 UD Merging between home and technion % 23.19 22.08.16 UD Anchor alignment tool continued % 23.18 10.08.16 UD Anchor alignment tool, X for Fadi. ROI Counter fix. % 23.17 05.07.16 UD Testing for Fadi - Cross correlation. % 23.16 14.06.16 UD Data management file for Prarie directory. Matrix registration improved. % 23.15 31.05.16 UD Releasing Prarie directory. % 23.14 21.05.16 UD Improving ROI snap to fluorescence. Add Effective ROI contour to ROI manager. % 23.13 03.05.16 UD Manual Spikes editor save is fixed. % 23.12 19.04.16 UD Manual Spikes editor has meaningful height. % 23.11 05.04.16 UD Changing dF/F computation in TP viewer. Changing dF/F bias term to 10. % 23.10 29.03.16 UD Spike detector editor. % 23.09 21.03.16 UD Working to snap ROIs to cells. % 23.08 15.03.16 UD Yael bug resolved. % 23.07 01.03.16 UD Small problems in Multi Group Views. % 23.06 23.02.16 UD Working with delays and spikes % 23.05 17.02.16 UD Adding Maria graphs % 23.04 16.02.16 UD Merging with technion version, fixing bugs % 23.03 14.02.16 UD Adding Maria sort view and save spikes per ROI from Editor % 23.02 06.02.16 UD Debug roi class % 23.01 26.01.16 UD Merging with 2204 and also continue to debug % 23.00 19.01.16 UD Debugging Porting Rois to Class % 22.03 12.01.16 UD Porting Rois to Class % 22.02 12.01.16 UD Bugs in Liora Prarie flow % 22.01 05.01.16 UD Liora place % 22.00 05.01.16 UD Rearranging electro phys management path. Supports piezo angle decode. % 21.23 04.01.16 UD Liora Load and Bug Merge, Classifier Editor, etc % 21.22 29.12.15 UD Working on Prarie Stimulus load,Overlay of Spikes and Event Sequence filter. % 21.21 15.12.15 UD Integrating with Previous version of the Prarie Stimulus load. Fixes in spike display. % 21.20 10.12.15 UD fixing bugs liora. % 21.19 08.12.15 UD PSTH analysis bug fixing. Adding dF/F with bias. % 21.18 01.12.15 UD Testing with Liora % 21.17 20.11.15 UD Bugs solving - Jackie % 21.16 26.11.15 UD z-stack fixes. recheck ROIs % 21.15 24.11.15 UD Fixing ROI assign bug % 21.14 24.11.15 UD Event Order additions. MTExplorer Config Spike detect. % 21.13 24.11.15 UD Tool for ROI movement - rot and scale. % 21.12 18.11.15 UD Fixing prarie load. % 21.11 17.11.15 UD Comments on ROI naming. % 21.10 10.11.15 UD Fixing empty prarie directories. % 21.09 03.11.15 UD Working with Liora on Bugs. % 21.08 20.10.15 UD Fixing bugs for prarie system. % 21.07 13.10.15 UD Event detection - fixes. % 21.06 10.10.15 UD Event detection - manula configuration for Itsik. % 21.05 08.09.15 UD Which score to import for Maria - predicted or manual. % 21.04 25.08.15 UD ROi artifact removal for Itsik. % 21.03 25.08.15 UD Shifting ROIs according to Z stack. % 21.02 18.08.15 UD Mergin event class to behavior editor from 2101. Moving files around. % 20.13 18.08.15 UD Categorization of the ROIs. % 20.12 28.07.15 UD Session : save / load experiment data. Cross probing in MultiTrial. % 20.11 28.07.15 UD Testing and doing video analysis. File CSV debug. % 20.10 24.07.15 UD Merging % 20.09 21.07.15 UD Skipping previous versions. MAC xls save resolve. % 20.08 15.07.15 UD Overlay TP on Video data. % 20.07 28.05.15 UD Fixing delay for continuous traces. % 20.06 22.05.15 UD Trajectory is differential. Fixing Prarie interface. % 20.05 19.05.15 UD Checking if it is possible do not use EventList object. % 20.04 17.05.15 UD Merging with 2003 - continuous events. % 19.32 10.05.15 UD Optical flow visualization. % 19.31 07.05.15 UD Testing and working on complex events. % 19.30 05.05.15 UD Debugging traces. Func Reconstruction. % 19.29 01.05.15 UD Working on complicated search events and addind cell functional reconstruction tool. % 19.28 24.04.15 UD Creating complicated search events. Fixing Time when Number of Slices is used % 19.27 21.04.15 UD Working with Maria and fixing bugs % 19.26 18.04.15 UD ToDo list: ROI from different z stacks % 19.25 10.03.15 UD Bug fixing - integration with Prarie. % 19.24 23.02.15 UD Working on trajectory integration. Adding Event management in XY view. % 19.23 17.02.15 UD Testing delay view. Integrating trajectories. % 19.22 12.02.15 UD Creating cell activity delay view. % 19.21 20.01.15 UD Problems with XLS dir structure to CSV - MAC compatability issues. MultiTrial - adding 2 cursors. % 19.20 13.01.15 UD Trying to do motion analysis. % 19.19 11.01.15 UD Merging 19.18 in the lab. Fixing bugs with multi stack registration. % 19.17 30.12.14 UD Trajectory for behavioral. Adding ROI position adjustment tool. % 19.16 23.12.14 UD Fixing dF/F and Event insertion when no events found. Group does not work. Multiplain registration. % 19.15 18.12.14 UD Adding dF/F for XY and Cursors % 19.14 18.11.14 UD Working on training and MultiGroupExplorer % 19.13 04.11.14 UD Multitrial explorer - adding color selection and save screeen as images as jpg/.... % 19.12 21.10.14 UD Merging with 1909. Fixes and bugs. % 19.11 16.10.14 UD Skipping version in the Lab. Adding group definition using Kulback-Leibler conditioned on event. % 19.08 07.10.14 UD Updates for Ronen - Event counting. Adding Tone/Table event insertion. Directory rename. % 19.07 03.10.14 UD Testing different bugs. Trying to load analysis data only. % 19.06 21.09.14 UD Adding import to movie_comb.avi file from new JAABA_multi SW % 19.05 11.09.14 UD Fixing bug with event load. Inconsistent file end fix. % 19.04 12.08.14 UD Continue to work on Adams interface. ROI selection is working. % 19.03 07.08.14 UD Adding check ShiftNum and Image Num % 19.02 05.08.14 UD Testing multiple stack ROIs - working. Trying to integrate with JAABA explorer. % 19.01 11.07.14 UD New data base management using excel. Skipping version 1900 that contains ElectroPhysiology IF Integration. % 18.13 10.07.14 UD Simon code for line correlation. Smart query for Explorer. % 18.12 09.07.14 UD Check Pointing. Adding Excel import tool with columns. % 18.11 08.07.14 UD Adding Multi Experiment Tool. procROI data disappears when you touch ROI. % 18.10 08.07.14 UD Check pointing. % 18.09 06.07.14 UD Back to Janelia. Analysis only support. % 18.08 24.06.14 UD Event auto detect continue. Try to compile the toolbox. B. Event bug fixes. % 18.07 20.05.14 UD trying to open old Two Phton data from the Lab % 18.06 14.05.14 UD ROI set movement in dx,dy % 18.05 30.04.14 UD Event auto detect. % 18.04 26.04.14 UD Behavior Event auto detect. read Jaaba Excel. Fix bug in Jaaba Load. % 18.03 25.04.14 UD Rename to Analysis. % 18.02 22.04.14 UD Testing and bringing debug info from m17.11. % 18.01 13.04.14 UD Integrating in one GUI. % 18.00 08.04.14 UD Adding ElectroPhysiology data analysis support . % 17.09 07.04.14 UD dF/F - 10% min and 10% min continuous. % 17.08 05.04.14 UD Reshecking ROI problems. Synthetic data gen. Bug found in ROI index exoprt. % 17.07 02.04.14 UD Adding debug info for jackie's auto detect error % 17.06 27.03.14 UD Auto Detect % 17.05 22.03.14 UD Cell Auto Detect and Multi Explorer Fixes % 17.04 21.03.14 UD Working on registration % 17.03 12.03.14 UD Checking registration % 17.02 10.03.14 UD Working on cell detection. MEanwhile all cells are projected on all times in all trials. % 17.01 06.03.14 UD Improve search in Trial Explorer. % 17.00 04.03.14 UD Changing ROI display.Send to Jackie. % 16.26 02.03.14 UD Working on file rename utility. Adding x pos indicators. % 16.25 27.02.14 UD Adding Ytzhak/Slice file load. % 16.24 27.02.14 UD Integrating pulse detection with dF/F. Fixing bugs % 16.23 26.02.14 UD Trying to resolve. % 16.22 26.02.14 UD Improvements in MultiTrial. Event problems are still not resolved. % 16.21 26.02.14 UD Session management is according to experiment. Counters are atatched to the session. % 16.20 26.02.14 UD Working on Explorer. Clear all at the start. % 16.19 25.02.14 UD Backup Jackie % 16.18 25.02.14 UD Still working on Multi dF/F and TrialExplorer. Fixing Z stack management and ROI over Z % 16.17 25.02.14 UD Adding Trial Explorer.Working on 2 Z stacks. Adding counter of rois and events. % 16.16 24.02.14 UD Improving user experience and fixing info delete. % 16.15 24.02.14 UD Starting Multi Trial % 16.14 23.02.14 UD ROI Analysis done % 16.13 23.02.14 UD Continue to TwoPhoton full analysis. Solving some Save and Load problems - TESTED OK % 16.12 22.02.14 UD Bringing in ROI class - small changes % 16.11 22.02.14 UD Testing. Working on Bugs % 16.10 21.02.14 UD Single ROI first - talking with Jackie. % 16.09 21.02.14 UD Check point. Tiff warning is fixed. 4 Windows are working for position. % 16.08 20.02.14 UD Window sync - not working good. % 16.07 20.02.14 UD rename views got confused of the naming. Adding resolution support. Registration is working. % 16.06 19.02.14 UD ROI management revisited. % 16.05 18.02.14 UD Fixing Jackies bugs. Working on sync. % 16.04 17.02.14 UD Check point. 1603 - Version for Jackie & Oded. Working on Sync issues. Adding Decimation and Registration. % 16.03 16.02.14 UD Check point. Adding data alignment GUI % 16.02 15.02.14 UD Restructuring.Time Explorer GUI created. % 16.01 14.02.14 UD Continue to develop. Changing period to repeat. % 16.00 13.02.14 UD Interface to Janelia Data. % 15.01 06.02.14 UD Working on Email comments. Editor from Liora TSeries. % 15.00 16.01.14 UD Integrating New ROI Editor. % 14.01 28.12.13 UD Improving browsing and ROI editing. % 14.00 22.12.13 UD Rename for Janelia only. Working on email notes. % 13.03 19.12.13 UD Testing. % 13.02 26.11.13 UD ROI and Janelia integration.. % 13.01 10.11.13 UD Rename again to TSeries and working on ROI. Adding janelia support. % 13.00 03.11.13 UD Rename AnalysisROI and adding new ROI management % 12.01 14.09.13 UD Continue on Z stack % 12.00 03.09.13 UD Working on Z Stack % 11.11 01.09.13 UD Align versions % 11.10 31.08.13 UD ROI Bugs % 11.09 20.08.13 UD More "Improvements" % 11.08 13.08.13 UD Improvements % 11.07 06.08.13 UD Red Channel % 11.06 06.08.13 UD bug Fix in ROI % 11.05 30.07.13 UD multiple sync options % 11.04 23.07.13 UD new day same sheet % 11.03 15.07.13 UD fixing bugs % 11.02 15.07.13 UD adding more features % 11.01 09.07.13 UD improving % 11.00 08.07.13 UD restructuring and major update % 10.11 07.07.13 UD Implementing Doc from Maria and Jackie % 10.10 02.07.13 UD Updating ROI processing % 10.09 30.06.13 UD ROI handling cont % 10.08 29.06.13 UD Big ROI data filtering % 10.07 25.06.13 UD interfaces updates % 10.06 25.04.13 UD Image load stack - T is the last dimension % 10.05 23.04.13 UD Improving 3D representation % 10.04 08.04.13 UD Multiple ROI and Z stack handling % 10.03 12.03.13 UD Farther development % 10.02 12.03.13 UD Adding GUI to explore the dataset %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
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Behavioral and Two Photon data analysis tools which include ROI selection, Data processing, Analysis and Exploration
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