REBOSEP is a workflow designed for detecting gene expression patterns across tissue boundaries using spatial transcriptomics data. REBOSEP supports tissue boundary identification and reports gene expression as a function of boundary distance. We have included a resource of gene expression patterns across five different brain-meninges boundaries in the developing mouse brain: dorsal pallium - meninges, midbrain - meninges, lower hindbrain - meninges, upper hindbrain - meninges, subpallium - meninges.
The gene expression profiles of five boundary regions can be downloaded from resource.
REBOSEP can be installed using pip:
pip install rebosep
An example workflow including a small dataset for testing purposes.
The scripts used to create the results presented in the publication.